• Bengaluru
  • 17 May 2016
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  • By Ms Namritha Ravinder , Senior R&D Manager, Synthetic biology, Thermo Fisher Scientific

CRISPR-Cas9 genome editing—leading in engineering, inspiring design, and empowering discovery

CRISPRs and CRISPR-associated (Cas) proteins are found in many bacteria and most archaea

namritha-ravinder

Ms Namritha Ravinder , Senior R&D Manager, Synthetic biology, Thermo Fisher Scientific

With increasing expansion into research areas of more biological relevance, existing molecular and cellular techniques need to be improved. The area of genome editing is rapidly growing and requires more advanced techniques to maximize its potential applications. Transcriptional activator-like effector nucleases (TALENs) and technology derived from clustered regularly interspaced short palindromic repeats (CRISPRs) allow precise cleavage of DNA at specific loci. However, the effectiveness of these tools is contingent upon the intrinsic properties of the locus of interest, efficient delivery, and the painstaking downstream processes of generating stable cell lines and knockout models to study the phenotypic effects of such genetic modifications.

CRISPRs and CRISPR-associated (Cas) proteins are found in many bacteria and most archaea. The CRISPR-Cas systems use sequences derived from plasmids and phages to activate Cas endonucleases to neutralize those plasmids and phages via RNA-guided sequence-specific DNA cleavage, thus blocking their transmission and creating a simple acquired immunity.

With their highly flexible but specific targeting, CRISPR-Cas systems can be manipulated and redirected to become powerful tools for genome editing. CRISPR-Cas technology permits targeted gene cleavage and gene editing in a variety of eukaryotic cells, and because the endonuclease cleavage specificity in CRISPR-Cas systems is guided by RNA sequences, editing can be directed to virtually any genomic locus by engineering the guide RNA sequence and delivering it along with the Cas endonuclease to a target cell.

Thermo Fisher Scientific provides an easy-to-use, optimized and validated genome editing solution, designed to expedite life science research. The Thermo Fisher Scientific solution spans the entire cell engineering workflow, making genome editing accessible to anyone at any level. And Thermo Fisher will continue to expand its suite of genome editing products, from cell culture and delivery reagents and sample preparation, to genome modification, detection and analysis of known genetic variants.

An online CRISPR search and design tool along with CRISPR-Cas9 is available to researchers in four formats: an all-in-one expression vector, Cas9 mRNA, Cas9 protein, and CRISPR libraries services. These gene editing solutions are paired with the optimal cell culture reagents, delivery method, and analysis tools based on required application and cell type.

 

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